Molecular surveillance of anti-malarial resistance pfcrt, pfmdr1, and pfk13 polymorphisms in African Plasmodium falciparum imported parasites to Wuhan, China

Abstract Background Imported malaria parasites with anti-malarial drug resistance (ADR) from Africa is a serious public health challenge in non-malarial regions, including Wuhan, China. It crucial to assess the ADR status African Plasmodium falciparum isolates imported cases, as this will provide valuable information for rational medication and control. Methods During 2017–2019, cross-sectional study was carried out Peripheral blood 3 ml of returned migrant workers collected. The target fragments pfcrt , pfmdr1 k13 propeller ( pfk13 ) genes were amplified, sequenced, analysed. Results In total, 106 samples Subsequently, 98.11% (104/106), 100% (106/106), 86.79% (92/106) these successfully amplified sequenced (72–76), genes, respectively. prevalence 76 T 86 Y 184 F mutations 9.62, 4.72, 47.17%, At codons 72–76, locus displayed three haplotypes, CVMNK (wild-type), CV IET (mutation type), M/ I N/ E K/ (mixed 87.50%, 9.62%, 2.88% prevalence, For gene, NY (wild N YF (mutant Y/ type) accounted 34.91, 43.40, 3.77, 15.09, 0.94, 1.89% A total 83 six unique haplotypes found combined which NY-CVMNK -CVMNK 40.96% (34/83) 43.37% (36/83), Furthermore, 90 cases (84.91%, 90/106) at loci 93, 97, 101, 145, 78 (73.58%, 78/106) 343, 353, 356 . However, mutation observed only 6.41%. reported Southeast Asia (at 474, 476, 493, 508, 527, 533, 537, 539, 543, 553, 568, 574, 578, 580) 550, 561, 575, 579, 589) not observed. Conclusions present data demonstrate that drugs chloroquine, amodiaquine, mefloquine, remain effective against treatment Africa. new related piperaquine relatively low levels. Another source concern artemether-lumefantrine resistance-related profiles N86 Although no detected, molecular surveillance must continue.